Figure 4From: Differences in virulence of pneumolysin and autolysin mutants constructed by insertion duplication mutagenesis and in-frame deletion in Streptococcus pneumoniaeVerification of D39 and the IFD- ply and - lytA mutants. PCR using different primer sets specific to ply and lytA were used to confirm parental D39 and the IFD-ply and -lytA mutants. The locations of primers and predicted molecular weights of the PCR products for D39, IFD-ply, and IFD-lytA are given in Figure 3 and Table 1. M, molecular DNA marker.Back to article page