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Figure 1 | BMC Biotechnology

Figure 1

From: Differences in virulence of pneumolysin and autolysin mutants constructed by insertion duplication mutagenesis and in-frame deletion in Streptococcus pneumoniae

Figure 1

Construction of the ply and lytA knockdown mutants by IDM. Partial sequences of pneumolysin (ply-s) (A) and autolysin (lytA-s) (B) genes were cloned into plasmids pVA891 (A) and pEVP3 (B), containing an erythromycin (erm)- and a chloramphenicol (cat)-resistance marker, respectively. Cloned plasmids were then used to generate homologous recombinants from their WT ply or lytA genes, respectively. Two primer sets, ply-P1/pVA891-R and ply-P2/pVA891-F or lytA-P1/pEVP3-R and lytA-P2/pEVP3-F, were used to verify the insertion of pVA891 and pEVP3 in IDM-ply (A) and -lytA (B), respectively. C, ClaI.

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BMC Biotechnology

ISSN: 1472-6750

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