Table 5 Plasmids and strains used in this study

S. cerevisiae

CEN.PK113-5D

MATa SUC2 MAL8C ura3-52

Peter Kötter

BSPX042

CEN.PK113-5D, XKS1::loxP-TEF1p, gre3(-241, +338) ::TPI1p-RKI1-RKI1t-PGK1p-TAL1-TAL1t-FBA1p-TKL1-TKL1t-ADH1p-RPE1-RPE1t-loxP, cox4::loxP, adaptive evolution, ura3-52

[28]

BSLS000

BSPX042, leu2::loxp-KanMX-loxp

This study

XICO

BSLS000, pJX7&pYX242-WS

This study

XITN

BSLS000, pJX7&pYX242-TEF1nox

This study

XIGN

BSLS000, pJX7&pYX242-GPD2nox

This study

XRCO

BSLS000, pJX1&pYX242-WS

This study

XRTN

BSLS000, pJX1&pYX242-TEF1nox

This study

XRGN

BSLS000, pJX1&pYX242-GPD2nox

This study

XRHN

BSLS000, pJX1&pYX242-HXK2nox

This study

XRTN2

BSLS000, pJX1&pRS315-TEF1nox

This study

Plasmids

pYX242-WS

2 μ plasmid with TEF1 promoter and Poly A terminator, LEU2 marker

This study

pRS315

Centromeric plasmid with LEU2 marker

ATCC77144

pYX242-TEF1nox

pYX242-WS, noxE gene with TEF1 promoter, LEU2

This study

pYX242-GPD2nox

pYX242-WS, noxE gene with GPD2 promoter, LEU2

This study

pYX242-HXK2nox

pYX242-WS, noxE gene with HXK2 promoter, LEU2

This study

pRS315-TEF1nox

pRS315, noxE gene with TEF1 promoter, LEU2

This study

pJX1

YCplac33, XYL1 with TEF1 promoter, XYL2 with TDH3 promoter

[12]

pJX7

YEplac195, 2 μ, Ru-xylA with TEF1 promoter, URA3

[28]