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Table 4 Primers used in this study

From: Fine-tuning of NADH oxidase decreases byproduct accumulation in respiration deficient xylose metabolic Saccharomyces cerevisiae

Primer name

Sequence (5′ → 3′)

Template

Purpose

Leu2 up

ATGTCTGCCCCTAAGAAGATCGTCGTTTTGCCAGGTGACAGCTGAAGCTTCGTACGCTG

pUG6

LEU2 gene deletion

Leu2 down

CACCAGTTCTGATACCTGCATCCAAAACCTTTTTAACTGATAGGCCACTAGTGGATCTG

pUG6

LEU2 gene deletion

TEF1W up

CCCAAGCTTCACAATGCATACTTTGTACGTTa

BSPX042 chromosome

pYX242-WS

TEF1W down

GCGCGTCGACTTGTAATTAAAACTTAGATTAGb

BSPX042 chromosome

pYX242-WS

noxE up

ACGCGTCGACATGAAAATCGTAc

L. lactis chromosome

pYX242- TEF1nox

noxE down

GTCCGAGCTCTTATTTGGCATTCd

L. lactis chromosome

pYX242- TEF1nox

GPD2p up

TTTAATAACTCGAAAATTCTGCGTTCGTTAAAGCTTCGACATATCTATTATAGTGGGGAGA

BSPX042 chromosome

pYX242- GPD2nox

GPD2p down

GCCTGCGTGGTTTGTACCGATAACTACGATTTTCATGTCGACACGACTAGTGACAGCAAGCATT

BSPX042 chromosome

pYX242- GPD2nox

HXK2p up

TAAGTTTAATAACTCGAAAATTCTGCGTTCGTTAAAGCTTTTGAAAAAAAGTGCGGGGC

BSPX042 chromosome

pYX242-HXK2nox

HXK2p down

CTGCGTGGTTTGTACCGATAACTACGATTTTCATGTCGACTTTATTTAATTAGCGTACT

BSPX042 chromosome

pYX242-HXK2nox

TEF1p up

TGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCACAATGCATACTTT

BSPX042 chromosome

pRS315-TEF1nox

TEF1p down

GGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTAGCCGGCGAACGTGGC

BSPX042 chromosome

pRS315-TEF1nox

ACT1qPCR up

CAAACCGCTGCTCAATCTTC

cDNA

qPCR

ACT1qPCR down

AGTTTGGTCAATACCGGCAG

cDNA

qPCR

NOXqPCR up

TACTGCCAACAGTGCCTTGG

cDNA

qPCR

NOXqPCR down

TTCCTGACCGAACAGCGTTT

cDNA

qPCR

  1. aunderline: Hind III restriction site used for ligation.
  2. bunderline: SalI restriction site used for ligation.
  3. cunderline: SalI restriction site used for ligation.
  4. dunderline: SacI restriction site used for ligation.