Primer name | Sequence (5′ → 3′) | Template | Purpose |
---|---|---|---|
Leu2 up | ATGTCTGCCCCTAAGAAGATCGTCGTTTTGCCAGGTGACAGCTGAAGCTTCGTACGCTG | pUG6 | LEU2 gene deletion |
Leu2 down | CACCAGTTCTGATACCTGCATCCAAAACCTTTTTAACTGATAGGCCACTAGTGGATCTG | pUG6 | LEU2 gene deletion |
TEF1W up | CCCAAGCTTCACAATGCATACTTTGTACGTTa | BSPX042 chromosome | pYX242-WS |
TEF1W down | GCGCGTCGACTTGTAATTAAAACTTAGATTAGb | BSPX042 chromosome | pYX242-WS |
noxE up | ACGCGTCGACATGAAAATCGTAc | L. lactis chromosome | pYX242- TEF1nox |
noxE down | GTCCGAGCTCTTATTTGGCATTCd | L. lactis chromosome | pYX242- TEF1nox |
GPD2p up | TTTAATAACTCGAAAATTCTGCGTTCGTTAAAGCTTCGACATATCTATTATAGTGGGGAGA | BSPX042 chromosome | pYX242- GPD2nox |
GPD2p down | GCCTGCGTGGTTTGTACCGATAACTACGATTTTCATGTCGACACGACTAGTGACAGCAAGCATT | BSPX042 chromosome | pYX242- GPD2nox |
HXK2p up | TAAGTTTAATAACTCGAAAATTCTGCGTTCGTTAAAGCTTTTGAAAAAAAGTGCGGGGC | BSPX042 chromosome | pYX242-HXK2nox |
HXK2p down | CTGCGTGGTTTGTACCGATAACTACGATTTTCATGTCGACTTTATTTAATTAGCGTACT | BSPX042 chromosome | pYX242-HXK2nox |
TEF1p up | TGGAGCTCCACCGCGGTGGCGGCCGCTCTAGAACTAGTGGATCCCACAATGCATACTTT | BSPX042 chromosome | pRS315-TEF1nox |
TEF1p down | GGGTACCGGGCCCCCCCTCGAGGTCGACGGTATCGATAAGCTTAGCCGGCGAACGTGGC | BSPX042 chromosome | pRS315-TEF1nox |
ACT1qPCR up | CAAACCGCTGCTCAATCTTC | cDNA | qPCR |
ACT1qPCR down | AGTTTGGTCAATACCGGCAG | cDNA | qPCR |
NOXqPCR up | TACTGCCAACAGTGCCTTGG | cDNA | qPCR |
NOXqPCR down | TTCCTGACCGAACAGCGTTT | cDNA | qPCR |