Fine-tuning of NADH oxidase decreases byproduct accumulation in respiration deficient xylose metabolic Saccharomyces cerevisiae

Table 3 Physiological parameters of recombinant S. cerevisiae strains from batch cultivations on glucose and xylose

Strain	μ_max(h^-1)^a	Consumed xylose in 78 h (g L^-1)	Sugar consumption rate (g L^-1 h^-1)^b		Yield on sugars (g g^-1)^c			Xylitol yield (g g^-1)^d	Carbon balance
			Glucose	Xylose	Biomass	Ethanol	Glycerol
XRCO	0.17	18.53	1.37	0.37	0.085	0.35	0.121	0.53	0.90
XRGN	0.18	18.47	1.39	0.37	0.094	0.37	0.035	0.45	0.86
XRTN2	0.16	13.79	1.20	0.29	0.080	0.36	0.023	0.21	0.82
XRHN	0.17	10.69	1.18	0.24	0.098	0.35	0	0.03	0.91
XRTN	0.15	3.31	0.98	0.08	0.094	0.29	0	0	0.84

Shown are the average values from independent duplicate cultivations. In all cases, the standard error is less than 3%.
^a Maximum specific growth rate (h^-1).
^b Specific volumetric rate of glucose or xylose (g L^-1 h^-1).
^c Biomass, ethanol or glycerol yield from consumed sugars (g g^-1 sugar).
^d Xylitol yield on xylose (g g^-1 xylose).

ISSN: 1472-6750