Figure 2

Spectral unmixing allows dynamic, medium throughput quantification of levels of proteins. Cells were grown in 3% glycerol, transferred into media with 3% glycerol and 2% glucose, and followed in the plate reader with data taken every 12 minutes. Genes are ordered by maximum level of expression with this maximum decreasing across the rows and down each column (Hxk1p has the highest maximum expression; Pol32p has the lowest maximum expression). Fluorescence values are in arbitrary units, with long tick marks showing a change in fluorescence of a factor of 10. The y-axes in the first two rows ranges from 10³ to 10⁴ units. The blue curves are protein levels per cell for genes with increased mRNA expression from 0-1 hours, using the data from Wang et al.[19]; the green curves are protein levels per cell for genes with decreased mRNA expression from 0-1 hours. Cell density initially increases and then plateaus at approximately 12 hours. Error bars are shown with a dashed line (Methods).