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Table 2 The 8 bp window motif analyses for several DNA polymerase experiments

From: DNA polymerase preference determines PCR priming efficiency

Top 1% (655 in 65,536)

8 bp window motif analyses for several DNA polymerase experiments

 

QTT-A

QTT-AR

PGT-A

PGT-AR

QTT-A63

QTT-A65

QTT-A67

QHH-B

(GC,CG) motif at 3’ end of primer

88%

89%

90%

90%

90%

91%

88%

25%

(GC,CG) motif at 5’ end of 6 N portion of the primer

8%

6%

8%

11%

12%

10%

8%

89%

(TC) motif at first 2 bp of runway

3%

4%

3%

3%

4%

4%

4%

19%

(CC) motif at first 2 bp of runway

28%

26%

28%

30%

23%

23%

25%

30%

(CGC,CCG,GCG, GGC,TGC,TCG) motif at 3’ end of primer

87%

88%

88%

89%

90%

90%

87%

22%

(AGC,ACG) motif at 3’ end of primer

1%

1%

1%

1%

1%

1%

1%

4%

(ACG) motif at 3’ end of primer

1%

1%

1%

1%

1%

1%

1%

2%

(AGC) motif at 3’ end of primer

0

0

0

0

0

0

0

2%

  1. The motif percentage breakdown for the top 1% of ranked sequences are shown for several PCR experiments: QTT-A touchdown, QTT-AR, PGT-A, PGT-AR, QTT-A63, QTT-A65, QTT-A67, and the QHH-B touchdown PCR amplification experiment.