Figure 2
From: DNA polymerase preference determines PCR priming efficiency
Observation windows for the 12 N portion of the SL. (A) The 4 bp window includes the nucleotides 4 bp ahead of the primer:template junction; (B) the 6 bp window includes the primer:template interaction; (C) the 8 bp window includes the primer:template interaction and 2 bp ahead of the primer:template junction; and (D) the 10 bp window includes the primer:template interaction and 4 bp ahead of the junction.