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Table 2 Effects of variables on cell growth, rPly activity and process productivity

From: Experimental design approach in recombinant protein expression: determining medium composition and induction conditions for expression of pneumolysin from Streptococcus pneumoniae in Escherichia coliand preliminary purification process

 

Cell growth

rPly activity

Process productivity

 

Effect

p-value

Effect

p-value

Effect

p-value

Mean/interc.

3.82

<0.0001

985.4

<0.0001

2.57

<0.0001

Induction absorbance

1.43

<0.0001

323.5

0.0016

0.33

0.2248

IPTG

-0.42

0.0387

-52.0

0.5422

-0.19

0.4720

Expression temperature

1.13

<0.0001

-340.8

0.0011

-0.91

0.0041

Yeast extract

0.86

0.0004

77.0

0.3706

0.23

0.3930

Tryptone

0.67

0.0027

268.2

0.0061

0.79

0.0095

Glucose

-0.33

0.0920

164.3

0.0685

0.37

0.1797

Glycerol

-0.32

0.1011

44.8

0.5993

0.09

0.7241

Kanamycin

0.31

0.1163

256.0

0.0082

0.72

0.0160

Curvature

-0.40

0.2356

950.5

<0.0001

2.85

<0.0001

  1. In bold variables with statistical significance with a confidence level of 90% (p-value < 0.1).
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BMC Biotechnology

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