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Figure 5 | BMC Biotechnology

Figure 5

From: Simplified production and concentration of lentiviral vectors to achieve high transduction in primary human T cells

Figure 5

High transduction of lentivirus in primary human T cells. Primary CD45RA+ T cells were transduced using the ‘final’ simplified lentivirus generation and concentration protocol in combination with modifications made to the culturing conditions. Left panel shows representative histogram from one donor of NGFR expression following transduction with lentivirus. Right panel shows the percentage NGFR expression from three lentivirus preparations in nine independent experiments. Transduction efficiency was determined 24 hrs post-transduction using flow cytometry to determine the percentage of NGFR expression.

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BMC Biotechnology

ISSN: 1472-6750

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