Figure 3

Ultracentrifugation at 20 000 g improves virus titre and transduction efficiency in primary CD45RA⁺T cells. A. Virus titre measured before and after concentration by ultracentrifugation. B. The level of transduction using un-concentrated and concentrated lentivirus supernatant as measured by flow cytometry. C. Virus titre measured following centrifugation of viral supernatants at decreasing centrifugal speeds. D. Assessment by flow cytometry of transduction by lentivirus following concentration with decreasing centrifugal speeds. E. Virus titre measured following concentration of lentivirus using ultracentrifugation, Optiprep and Acrodisc methods. F. The level of transduction of each of the concentrated lentivirus stocks as measured by flow cytometry. *P > 0.05, **P > 0.01, ***P > 0.001 as determined by one-way ANOVA with Bonferroni correction or Students t-test. Data is represented as mean ± s.d. of three independent experiments and virus preparations.