Figure 5

Cyclic nucleotide induced luciferase activity in E. coli. BL21-AI cells transformed with a promoter luciferase reporter construct and treated with different concentrations of 8-bromo cGMP and dibutyryl cAMP. At least 3 separate colonies were tested for each promoter / treatment combination. (A) Effect of different concentrations of 8-bromo cGMP on B21A-AI cells transformed with OPTXcGMPRE:LUC (n = 3–4); (B) Effect of dibutyryl cAMP on B21A-AI cells transformed with OPTXcGMPRE:LUC (n = 3–4); (C) Effect of different concentrations of 8-bromo cGMP on B21A-AI cells transformed with OPTX-GARE:LUC (n = 4). (D) Effect of dibutyryl cAMP on B21A-AI cells transformed with OPTXGARE:LUC (n = 6). Luciferase activity was expressed relative to OD₆₀₀ to normalize results per cell number and asterisks indicate treatments significantly different from the control (P < 0.05; one way ANOVA, Dunnett’s multiple comparison post-test).