Skip to main content
Figure 3 | BMC Biotechnology

Figure 3

From: A cyclic nucleotide sensitive promoter reporter system suitable for bacteria and plant cells

Figure 3

Effect of cGMP on the luciferase reporter constructs in plant protoplasts. (A) Detection of CHX21, SOS3 and OPTX transcripts in Arabidopsis leaf mesophyll protoplasts. Control amplifications were also made of the UBQ10 gene from RNA samples with (+, plus) or without (−, minus) reverse transcriptase (RT) for protoplasts (OPTX, SOS3 and CHX21) or leaf tissue (SOS3 and CHX21 only) and M refers to the lane containing the DNA size ladder. (B-D) Arabidopsis leaf mesophyll protoplasts were transiently transfected with a promoter luciferase reporter construct (B: SOS3:LUC (n = 4 – 5); C: CHX21:LUC (n = 3 – 4); D: OPTX:LUC (n = 3–6)) and treated with 0, 0.1, 1 or 10 μM 8-bromo cGMP. At least 3 biological replicates were completed on different days for each promoter treatment combination. Luciferase was normalized against protein content and asterisks indicate treatments significantly different from the control (P < 0.05; one way ANOVA, Dunnett’s multiple comparison post-test).

Back to article page