Table 1 The relationship between culture conditions and viability (survived juveniles/ total eggs)
From: Development of a microinjection system for RNA interference in the water flea Daphnia pulex
160 mM (FW) | 80 mM (FW) | 60 mM (FW) | 40 mM (FW) | 0 mM (FW) | |
|---|---|---|---|---|---|
Glass petri dish | 0% (0/34) | 13.9% (5/36) | 45.9% (17/37) | 54.1% (20/37) | 69.6% (48/69) |
Plastic petri dish | 0% (0/56) | 8.5% (5/59) | 48.6% (35/72) | 59.5% (25/42) | 64.8% (35/54) |
2% agar plate | 0% (0/40) | 17.9% (7/39) | 72.5% (29/40) | 65.3% (32/49) | 79.2% (38/48) |
60 mM (M4) | 40 mM (M4) | 0 mM (M4) | |||
Glass petri dish | 61.9% (26/42) | 77.1% (27/35) | 88.5% (31/35) | ||
Plastic petri dish | 62.7% (32/51) | 70.0% (28/40) | 86.8% (33/38) | ||
2% agar plate | 88.9% (40/45) | 90.9% (40/44) | 96.4% (53/55) |