Figure 6From: New colorimetric screening assays for the directed evolution of fungal laccases to improve the conversion of plant biomassScreening in solid format. Saccharomyces cerevisiae cells secreting different laccase mutants grown for 5Â days in SC plates supplemented with sinapic acid (A), syringaldehyde (B) or acetosyringone (C). The colored halo around the colonies demonstrated the secretion of laccase (1 and 2 correspond to HRPL mutants, and 3 to LRPL mutant) as compared with the negative control (C-).Back to article page