Figure 3
Validation of HTS colorimetric assays with S-phenolic compounds. Linearity of the assays based on the oxidation of acetosyringone (A) and syringaldehyde (B) after 5 h, and sinapic acid (C) after 2 h, using crude extracts from S. cerevisiae micro-cultures secreting 3A4 HRPL (triangles) or R2 LRPL (squares). Coefficient of variation (CV) of the HTS colorimetric assays based on the oxidation of acetosyringone (D) and syringaldehyde (E) and sinapic acid (F). Laccase activities from different replicates of the same clone are plotted in descending order.