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Figure 2 | BMC Biotechnology

Figure 2

From: New colorimetric screening assays for the directed evolution of fungal laccases to improve the conversion of plant biomass

Figure 2

Determination of conditions for the HTS assays with S-phenolic compounds. Oxidation rates of TvL (10 mU) for sinapic acid (A), syringaldehyde (B) and acetosyringone (C) measured at 512 nm, 370 and 520 nm, respectively; and color responses of the endopoint HTS assays with time using 250 ÎĽM of sinapic acid (D) and 2 mM of syringaldehyde (E) and acetosyringone (F) with 15 ÎĽL of crude extracts from S. cerevisiae micro-cultures secreting 3A4 HRPL. Each point represents the mean and SD derived from three independent experiments.

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