Figure 5
From: Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source
Schematic drawing for the construction of strain MUR24Pf. Plasmid pMUR50 possesses a modified chitinase construct together with the resistance cassette as used for the construction of plasmid pMUR47. After transformation with circular pMUR50 the intermediate mutant MUR24Pf_i was isolated and characterized. The negative selection with 6-methylpurine resulted in strain MUR24Pf with a modified version of the chitinase.