Figure 2
From: Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source
Schematic drawing for the construction of marker-less strain MUR27Pf. Plasmid pMUR37 was obtained by overlapping PCR. It contained 1-kb upstream and downstream regions of PF1950 and the resistance cassette TK0914 for selection with the antibiotics simvastatin. After transformation the intermediate mutant MUR27Pf_i was isolated and characterized. An additional negative selection with 6-methylpurine produced strain MUR27Pf with the marker-less deletion of PF1950 (shadowed in grey).