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Figure 1 | BMC Biotechnology

Figure 1

From: Genetic engineering of Pyrococcus furiosus to use chitin as a carbon source

Figure 1

Comparison of chitinases from P. furiosus and T. kodakarensis. (A) Schematic models of catalytic and chitin binding domains of both chitinases, the single enzyme from T. kodakarensis and the split version from P. furiosus. (B) Alignment of the amino acid sequences. In the case of P. furiosus the corresponding DNA sequence was modified by the deletion of the adenine at position 1006. The details of this region were shown in the box within the alignment. The upper part within the box shows the original DNA sequence of P. furiosus together with the corresponding amino acid sequence in the region of the putative nucleotide insertion of PF1234. The lower part shows the sequence with the deleted nucleotide. For sequence comparison, the amino acid sequence of the corresponding chitinase region of T. kodakarensis is also shown and identical amino acids between both, T. kodakarensis and P. furiosus, are shadowed in black. The position with the additional nucleotide is underlined. The putative stop codon is marked by an asterisk.

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