Table 2 Cleavage of β and α S1-casein substrates by the P. lautus CtpA determined by MALDI-TOF MS
Substrates | Monoisotopic mass of neutral peptide Mr (cal) | Sequences | Cleavage sites | Expect value |
|---|---|---|---|---|
β-casein | 1624.8308 | A↓RELEELNVPGEIVE | Ala15-Arg16 | 0.000038 |
1528.7926 | Q↓TQSLVYPFPGPIPN | Gln69-Thr70 | 0.0011 | |
1299.6863 | Q↓SLVYPFPGPIPN | Gln71-Ser72 | 0.00003 | |
876.4739 | V↓MGVSKVKE | Val107-Met108 | 0.027 | |
947.5287 | Q↓SVLSLSQSK | Gln175-Ser176 | 0.0015 | |
994.6175 | Q↓SKVLPVPQK | Gln182-Ser183 | 0.0027 | |
1459.8915 | E↓PVLGPVRGPFPIIV | Glu210-Pro211 | 0.0037 | |
1150.6863 | L↓GPVRGPFPIIV | Leu213-Gly214 | 0.0022 | |
1093.6648 | G↓PVRGPFPIIV | Gly214-Pro215 | 0.00011 | |
996.6120 | P↓VRGPFPIIV | Pro215-Val216 | 0.0061 | |
α S1-casein | 1467.7915 | L↓LRFFVAPFPEVF | Leu35-Leu36 | 0.016 |
|  | 1051.5379 | F↓FVAPFPEVF | Phe38-Phe39 | 0.0011 |
|  | 760.3967 | N↓ELSKDIG | Asn53-Glu54 | 0.0041 |
|  | 753.3769 | S↓AEERLH | Ser130-Ala131 | 0.00092 |
|  | 1157.5499 | A↓EERLHSMKE | Ala131-Glu132 | 0.000021 |
|  | 1107.5270 | N↓SEKTTMPLW | Asn205-Ser206 | 0.00045 |
|  | 1004.5001 | S↓EKTTMPLW | Ser206-Glu207 | 0.00015 |
|  | 875.4575 | E↓KTTMPLW | Glu207-Lys208 | 0.0026 |