Table 1 Sets of forward and reverse primers used in the experiments
Primers | Sequences | Annealing temperature (°C) | Amplicon size (bp) |
|---|---|---|---|
A1 (ASAL forward) | 5′ATGGCCAGGAACCTACTGACGA 3′ | 56 | 362 |
B1 (ASAL reverse) | 5′CTATCTTCTCTAGGTACCAGTA 3′ |  |  |
A2 (cre forward) | 5′ ATGTCCAATTTACTGACC 3′ | 58 | 1100 |
B2 (cre reverse) | 5′ CTAATCGCCATCTTCCAGC 3′ |  |  |
A3 (hpt forward) | 5′ GCTTTCCACTATCGGCGA 3′ | 58 | 980 |
B3 (hpt reverse) | 5′ AAAGCCTGAACTCACCGC 3′ |  |  |