PCR analysis of F
progeny plants. Ten randomly chosen seeds from LA2CC3(3) line were analysed through multiplex PCR using ASAL and cre gene specific primer pairs. Lane 1–10 represents plant no. LA2CC3(3) 1–10 respectively, lane '+’ represents mix of pBKhgASAL and pBK16.2 plasmids as positive control and lane '-’ represents negative control (genomic DNA of untransformed plant); lane M, DNA molecular weight marker (GenerulerTM, MBI Fermentas, UK).