Insect bioassay setup and effect of expressed ASAL on Lipaphis erysimi. A, B In planta insect bioassay setup of Lipaphis erysimi on transgenic mustard plants. Inset in A shows Lipaphis erysimi culture on control mustard plant. Bar represents 1 mm. C Graph shows the percentage of survival of mustard aphid on untransformed control plant and five ASAL expressing F1 hybrid plants LA2CC3 3, 9, 14, 18, 23. D Bar diagram showing fecundity pattern (mean number of nymphs produced per plant) of L. erysimi fed on control and five F1 hybrid plants- LA2CC3 3, 9, 14, 18, 23.