Agglutination and thermal stability assay of expressed ASAL protein from F
hybrid plants. A Rabbit erythrocytes incubated with: Panel I-V; Wells 3 to 7: total soluble protein extracted from ASAL expressing F1 hybrid plants namely LA2CC3 3, 9, 14, 18, 23 respectively in a twofold serial dilution started from 250 μg to 15.6 μg/ well; Panel I-V; Well 1: No protein control. Panel I-V; Well 2: protein from untransformed control plant. B Thermal Stability Assay: Rabbit erythrocytes incubated with total soluble protein (125 μg) from ASAL positive F1 hybrid plants [LA2CC3 3, 9, 14, 18, 23 in panel I-V respectively] pre-incubated at different temperatures as- 4°C, 25°C, 37°C, 55°C, 75°C and 95°C in Panel I-V; wells 2–7 respectively. Well 1 (I-V): No protein control (100 μl of 1.5% rabbit erythrocytes incubated without ASAL).