Figure 4
Southern blot analysis of Hin dIII digested genomic DNA from nine hpt negative F 1 hybrid lines. DNA from Gel blots were hybridized with probe for A 362 bp ASAL sequence B 1.1 kb cre sequence and C 980 bp hpt sequences. Lane '+’ represents in A PCR amplified ASAL gene, B PCR amplified cre gene, C PCR amplified hpt gene as positive control, lane ' – ' represents HindIII digested genomic DNA from control plants as negative control. Approximate molecular weight markers are indicated.