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Table 1 In vivo recombination activity after overexpression of integrases in E. coli BL21(DE3)

From: Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome

Integrase

μg tagged protein/ml1

% recombination2

Bxb1

ND

100

ND

100

BL3

ND

100

φR4

ND

100

A118

0.6

100

TG1

2.7

100

MR11

2.9

50

φ370

4.1

100

SPBc

9.0

100

TP901-1

32.3

100

φRV

55.9

75

FC1

74.7

100

K38

319.6

100

φBT1

350.4

100

φC31

758.2

100

  1. 1The amount of StrepII tagged integrase detected in a Western blot from 1 ml of culture.
  2. 2The % of plasmid extracted from the cultures that had undergone recombination.