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Figure 6 | BMC Biotechnology

Figure 6

From: Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome

Figure 6

Analysis of G418-resistant, gancyclovir-resistant, attR-, attL-clones generated by the Wβ and TP901 integrases. A. Ten G418-resistant, gancyclovir-resistant, attR-, attL- clones generated by one or other of these two integrases were analysed by PCR across the indicated sequences in each of the two reporter plasmids used in the transfection. The numbers below the plasmid maps indicate the PCR reactions assayed in the table. Primer sequences used for these assays are listed in the (Additional file 1: Table S7). B. Sequence analysis of damaged sites in the three indicated clones. The diagram shows the regions deleted in the remnant of the flanking arrays found in each of three clones.

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BMC Biotechnology

ISSN: 1472-6750

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