Skip to main content
Figure 3 | BMC Biotechnology

Figure 3

From: Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome

Figure 3

Comparing the activity of φC31 integrase on isolated attachment sites or attachment sites located in the multi-site arrays. A-E. Assays φC31 integrase activity on attachment sites in different configurations. The indicated plasmids containing either attB or attP sites were incubated in the absence or in the presence of 0 nM (lane 1), 100 nM (lane 2), 200 nM (lane 3), 350 nM (lane 4) and 700 nM (lane 5) φC31 integrase. Recombination reactions were digested with either HindIII or BamHI and the products size fractionated by agarose gel electrophoresis. F. The data shown in A-E were analyzed densitometrically and the fraction of the substrate converted into product calculated as described in the materials and methods. This comparative analysis was carried out in two independent experiments.

Back to article page