Skip to main content
Figure 2 | BMC Biotechnology

Figure 2

From: Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome

Figure 2

Assay system for integrases in mammalian cells. The reporter plasmid design (A) used to assay either site-specific deletion or integration promoted by serine integrases in vertebrate cells. In the deletion reporter construct, called attP array CCAG HyTK attB array, the counter selectable gene CCAG HyTK was placed between an array of attB sites (B) and an array of attP sites (C). In the integration or recombinase-mediated cassette exchange constructs, the docking construct, attP array CCAG HyTK attP array, had the CCAG HyTK gene flanked by two arrays of attP sites and the reporter construct, termed attB array CCAG neo attB array (D) contained the CCAG Neo gene conferring resistance to G418 flanked by arrays of attB sites. The integrase expression constructs are shown schematically in (E) each containing an int gene modified at the 5′ and 3′ ends to encode a StrepII tag and a nuclear localization signal, respectively, and placed down-stream of a CCAG promoter and upstream of an internal ribosome entry site and a dominant selectable marker conferring either zeocin or xanthine resistance (ecogpt).

Back to article page