Microcontact printed substrates demonstrate inhibition of Oli-neu process outgrowth by myelin. (A, B) CellTracker Green-positive Oli-neu cells plated onto DyLight549-conjugated-PLL lines alone (A) or covered with a myelin lawn (B). (C) In the presence of the myelin lawn, cells adhere to the PLL lines but exhibit a significant reduction in process outgrowth. (D) Microcontact printed myelin lines immunostained for myelin basic protein (MBP; red). (E) Control showing the lack of signal when the primary antibody was omitted. (F, G) CellTracker Green positive Oli-neu cells plated onto a PLL lawn printed with myelin lines. (G) Cells were treated with Y27632, a ROCK inhibitor. (H) Cells treated with Y-27632 show enhanced outgrowth compared to the untreated cells.