Figure 4

Adhesion of COS7 cells on micropatterned PLL. Schematic representation (A) of the three different conditions used: (1) low concentration unconjugated-PLL lawn (1 pg/mL; green) and high concentration DyLight549-conjugated PLL lines (1 mg/mL; red), (2) high concentration DyLight549-conjugated PLL lines (1 mg/mL; red), and (3) low concentration lawn of unconjugated-PLL (1 pg/mL; green). (B) Cells seeded onto the first substrate adhere to the low-concentration lawn of PLL avoiding the high-concentration red PLL lines. (C) Cells seeded on the second substrate in the absence of a low concentration PLL lawn adhere to high-concentration red PLL lines. (D) Cells seeded onto low concentration PLL lawn (1 pg/mL) adhere and display a typical flat morphology. COS7 cells were stained for F-actin. 10 μm wide lines separated by a pitch of 60 μm. (E) Quantification of COS7 cell area overlapping printed PLL lines under the conditions schematized in A.