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Table 1 3’-terminal sequence of replication products under potential TNT activity

From: Characterization of Norovirus RNA replicase for in vitro amplification of RNA

TNT activity

3’-terminal sequence of cRNA

Number of clones

TNT less

3’ CCCUUA--

11

TNT 1 nts (C)

3’ CCCCUUA--

3

TNT 2 nts (CC)

3’ CCCCCUUA--

3

TNT 3nts (CCC)

3’ CCCCCCUUA--

1

TNT 2nts (AA)

3’ AACCCUUA--

1

TNT 2nts (GG)

3’ GGCCCUUA--

1

Deletion, insertion

Others

2

  1. The result of 3’-terminal sequencing of amplified products (n = 22) in the reaction using Temp(GGG-CCC) shown in Figure 3, sampled at 240 min of incubation.