Primer extension replication with NV3Dpol. (A) Schematic illustration of the template-primer hybridization. NV3Dpol (the amount was not detected) was incubated with template-primer hybrid (initial concentration of 5 pmol and 10 pmol, respectively) (lane1) or RNA template (initial concentration of 5 pmol) (lane2) (reaction volume = 20 μL), analyzed on a non-denaturing 10% PAGE, visualized by SYBRgreenII staining (B) or FITC fluorescence (C). Both images were merged (D). E, T, P and TP indicate NV3Dpol, RNA template, RNA primer and template-primer hybrid, respectively. +/- corresponds to the presence or absence of substrates. M; 10 bp DNA step ladder (Promega).