Effect of the 3’-C-stretch of a long template for the initiation efficiency of RNA replication. Two kinds of RNA template (TD257-735 (A) and TD257-735g734c (B) (0.4 pmol) were incubated with NV3Dpol (8 pmol), respectively (reaction volume = 20 μL), and analyzed on a non-denaturing 5% PAGE. M; 100 bp DNA ladder marker (Promega). (C) The PAGE images were visualized by SYBRgreenII staining and quantified with reference to 0 min as the standard. The band intensities of amplified dsRNA were plotted. Closed circle; TD257-735. Closed square; TD275-735g734c.