Preparation of NV3Dpol. (A) SDS-PAGE analysis of NV3Dpol-strep synthesized in the cell-free translation system and purified with Strep-Tactin Superflow Plus purification system (Lane1), and BSA (Lane2). M; protein molecular marker (Precision Plus Protein™ Dual Color Standards, Bio-Rad). SDS-PAGE was detected with SYPROruby (Invitrogen) staining. White arrowheads indicate the proteins from BSA solution (TaKaRa) used as carrier protein. (B) Same as (A) except incorporation of FluoroTect™ GreenLys tRNA into NV3Dpol-strep during the cell-free translation reaction and detected under a fluorescence detection mode. Translation reaction was run with or without the NV3Dpol-strep mRNA.