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Table 1 Carotenoids in wild-type and transformant strains in ppm (μg per g of dry yeast)

From: Increase in the astaxanthin synthase gene (crtS) dose by in vivo DNA fragment assembly in Xanthophyllomyces dendrorhous

 

X. dendrorhousstrain [ppm (%)]

 

UCD 67–385

Xd_1H1S

Xd_2H2S

( DHS3/ DHS3)

( DHS3/dhs3:: hph+crtS)

(dhs3:: hph+crtS/dhs3:: hph+crtS)

Cultivation time (h)

25

50

96

25

50

96

25

50

96

Carotenoid:

         

Total carotenoids

22.70 ± 11.16

34.31 ± 15.87

189.32 ± 86.51

14.35 ± 11.71

64.21 ± 1.13

188.37 ± 6.05

12.98 ± 4.93

84.79 ± 28.83

197.85 ± 34.65

 

(100)

(100)

(100)

(100)

(100)

(100)

(100)

(100)

(100)

Beta-carotene

ND

4.37 ± 1.25

6.90 ± 0.57

ND

7.4 ± 0.01

3.93 ± 0.08

ND

2.36 ± 0.29

0.72 ± 0.22

(13)

(4)

(12)

(2)

(3)

(1)

Equinenone†

ND

1.36 ± 0.31

7.01 ± 0.46

ND

2.84 ± 0.01

3.84 ± 0.05

ND

0.54 ± 0.32

0.88 ± 0.03

(4)

(4)

(4)

(2)

(1)

(1)

OH-equinenone†

ND

4.65 ± 0.38

11.47 ± 1.10

ND

5.79 ± 0.01

7.29 ± 0.04

ND

8.85 ± 0.31

1.06 ± 0.16

(14)

(6)

(9)

(4)

(10)

(1)

Cantaxanthin†

ND

0. 46 ± 0.12

2.24 ± 0.90

ND

0.73 ± 0

1.41 ± 0.05

ND

0.39 ± 0.12

0.83 ± 0.21

(1)

(1)

(1)

(1)

(1)

(1)

Phoenicoxanthin†

0.46 ± 0.39

3.99 ± 0.37

28.41 ± 3.53

0.5 ± 0.41

6.65 ± 0.01

15.93 ± 0.01

ND

5.50 ± 0.41

11.08 ± 0.07

(2)

(12)

(15)

(3)

(10)

(8)

(6)

(6)

Astaxanthin†

21.97 ± 0.62

18.81 ± 1.77

128.04 ± 5.06

13.85 ± 0.41

38.05 ± 0.04

154.13 ± 0.09

12.98 ± 0

65.84 ± 0.81

179.21 ± 0.52

(97)

(55)

(68)

(97)

(59)

(82)

(100)

(78)

(96)

Lycopene

ND

ND

ND

ND

ND

ND

ND

ND

0.51 ± 0.15

(1)

Neurosporene

ND

0.10 ± 0.04

ND

ND

0.54 ± 0.01

ND

ND

ND

ND

(0)

(1)

Torulene*

ND

0.07 ± 0.04

ND

ND

0.206 ± 0.01

ND

ND

ND

0.45 ± 0.03

(0)

(1)

(1)

OH-keto-torulene†*

ND

0.47 ± 0.20

0.76 ± 0.52

ND

1.72 ± 0.02

1.85 ± 0.02

ND

0.95 ± 0.24

1.49 ± 0.26

(1)

(1)

(2)

(1)

(1)

(1)

  1. ND: Not detected. Table values correspond to the average result from three independent cultures ± standard deviations. Percentage relative to total carotenoids is indicated in parentheses. †: hydroxy or/and keto carotenoid, *: monocyclic carotenoid.
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BMC Biotechnology

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