Figure 3

Assembly and integration of hygromycin B resistance and crtS gene expression cassettes into the DHS3 locus of X. dendrorhous by DNA assembler. Four DNA fragments: DHS3 “up”, hph expression cassette (composed by EF-1α promoter, hph gene and gpd terminator), crtS gene expression cassette (composed by EF-1α promoter, crtS gene and actin transcription terminator) and DHS3 “down” were amplified by PCR to direct the integration of exogenous genes into the DHS3 locus of the X. dendrorhous genome. Arrows represent the primers used (Additional file 1: Table S1).