Table 5 Nucleotide sequences of the primers used
Primer | Nucleotide sequence |
|---|---|
1 | 5′-CATGCCATGGGCATGGATTTTCTTCTTGGCATCAATT-3′, Tm=62.7°C |
2 | 5′-CCGCTCGAGAAAGTTCAGCAATTTGTACTCTTTG-3′, Tm=57.6°C |
3 | 5′-GGAATTCCATATGGATTTCCTGCTGGGTATTAACTACT-3′, Tm=62.0 |
4 | 5′-CCGCTCGAGGAAGTTCAGCAGCTTATACTCTTTC-3′, Tm=56.7 |