From: High-level expression of a novel thermostable and mannose-tolerant β-mannosidase from Thermotoga thermarum DSM 5069 in Escherichia coli
Primer
Nucleotide sequence
1
5′-CATGCCATGGGCATGGATTTTCTTCTTGGCATCAATT-3′, Tm=62.7°C
2
5′-CCGCTCGAGAAAGTTCAGCAATTTGTACTCTTTG-3′, Tm=57.6°C
3
5′-GGAATTCCATATGGATTTCCTGCTGGGTATTAACTACT-3′, Tm=62.0
4
5′-CCGCTCGAGGAAGTTCAGCAGCTTATACTCTTTC-3′, Tm=56.7