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Table 4 Characteristics of β-mannosidases from different sources for ap NPM as substrate

From: High-level expression of a novel thermostable and mannose-tolerant β-mannosidase from Thermotoga thermarum DSM 5069 in Escherichia coli

Strain

V max (μmol min-1mg-1)

K m (mM)

k cat (s-1)

k cat /K m (mM-1s-1)

K i for mannose (mM)

Optimal Temp (°C)

Reference

Thermotoga thermarum

227.27±1.59

4.36±0.05

1924.29±13.47

441.35±0.04

900

85

This work

Thermotoga neapolitana

36.9±2.5

3.1±2.5

c67.83

c24.23

bND

90

11

Thermotoga maritima

50.5

0.49

c8.43

c17.22

ND

95

10

Pyrococcus furiosus

31.1

0.79

31.1

40

ND

105

12

Thermoascus aurantiacus

c3.66

1.1

6.1

5.5

ND

76

17

Aspergillus niger

c30

0.3

c67.5

c225

ND

70

16

Thermobifida fusca

5.96

0.18

c9.34

c51.89

5.5

53

22

Aplysia kurodai

3.75

0.1

c6.25

c62.5

ND

40

3

  1. apNPM: p-nitrophenyl-β-D-mannopyranoside.
  2. bND: not determined.
  3. cCalculated by the data based on the reference.