High-level expression of a novel thermostable and mannose-tolerant β-mannosidase from Thermotoga thermarum DSM 5069 in Escherichia coli

Table 2 Specific activity of Tth Man5 β-mannosidase on various substrates

Substrate	Specific activity
	(μmol min^-1mg^-1)
p-nitrophenyl-β-D-mannopyranoside	102.00±2.65
1,4-β-D-mannan	89.50±1.34
galactan	ND
konjaku powder	ND
guar galactomannan	ND

ND: not detected. Values shown were the mean of triplicate experiments, and the variation about the mean was below 5%.

ISSN: 1472-6750