Figure 5From: High-level expression of a novel thermostable and mannose-tolerant β-mannosidase from Thermotoga thermarum DSM 5069 in Escherichia coliAnalysis of 1,4-β-D-mannan hydrolyzed by Tth Man5 β-mannosidase. The products of the reaction were examined with TLC. M: mannose. Lane 1, 2, 3: 1,4-β-D-mannan (5%, wt/vol) incubated with 4 μg Tth Man5 b-mannosidase in 200 μL 50 mM imidazole-potassium buffer (pH 5.5) for 0.5 h, 1 h and 2 h, respectively.Back to article page