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Figure 3 | BMC Biotechnology

Figure 3

From: Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia colivia a modified Sec-dependent transporter construct

Figure 3

Activation of STAT3 by E. coli derived viral IL-10. STAT3 phosphorylation (STAT3-pY705) was analyzed by immunoblot of protein extracts from human Daudi cells (HCMV IL-10; A) and J774.1 mouse macrophages (EBV IL-10; B) treated with different concentrations of bacteria-derived viral IL-10. Total STAT3 was used to ensure equal protein loading in all lanes (double bands in Daudi cells represent STAT3 isoforms α and β). Periplasmic and cytoplasmic fractions of pUC19 transformed E. coli BL21 (DE3) cells and commercial viral IL-10 proteins served as controls. One experiment representative of two is shown. PP = periplasmic fraction; CP = cytoplasmic fraction; Com. = commercial; Rec. = recombinant.

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BMC Biotechnology

ISSN: 1472-6750

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