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Table 1 Oligonucleotide sequences

From: Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V

Oligonucleotide

Sequence (5′ - 3′)

pUCamp_f

GT I CC TATGAGTAAACTTGGTCTGACAGTTACC

pUCamp_r

GT I CC GTCATCACCGAAACGCGCG

RFP-dev_f

TG I AG GCGCAACGCAATTAATGTGAG

RFP-dev_r

AC I TC GTTATTAAGCACCGGTGGAGTG

MITF_f

AG I TC ATGCTGGAAATGCTAGAATACAG

MITF_r

GT I CCTCA ACACGCATGCTCCGTTTCTTC

Mitf-FL_f

CATGCTAGCATGCTGGAAATGCTAGAATACAGTC

VR2_r

ATTACCGCCTTTGAGTGAGC

  1. Deoxyinosine residues are shown in bold, primer add-on tails are underlined and separated from regions complementary to the target sequences by spaces.
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BMC Biotechnology

ISSN: 1472-6750

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