Figure 6

Insert DNA fragment generation using a proofreading DNA polymerase. PfuTurbo C _x Hotstart polymerase was used for PCR amplification of insert DNA fragments using two deoxyinosine-containing oligonucleotides. Analytical agarose gel electrophoresis was performed with PCR products comprising the ampicillin resistance cassette (1114 bp, A), the mRFP1 reporter device (830 bp, B), and the Mitf coding sequence (1270 bp, C). Annealing temperatures which were used for PCR cycling are indicated relative to T_m for each lane.