Figure 4From: Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease VmRFP1-positive clones obtained by cloning an RFP-coding device into pUC19. Recombinant E. coli colonies expressing mRFP1. Detection was facilitated via excitation at 505Â nm. Manual counting yielded a positive fraction (dark blue markers) of about 92.4% (233 of 252 colonies), cyan markers present negative colonies with poor or no fluorescence.Back to article page