Dynamic strain scanning optimization: an efficient strain design strategy for balanced yield, titer, and productivity. DySScO strategy for strain design

Table 1 Knockout strategies for succinate overproduction identified in this work

Succinate Strains	YZ1	YZ2	YZ3
Growth rate (hr^-1)	0.16	0.24	0.21
Product yield	1.27	0.89	0.92
(mol/mol glc)	ALCD2x	F6PA	ACALD
Knockouts	GLUDy	G6PDH2r	F6PA
	LDH_D	ME2	G6PDH2r
	PPKr	MTHFD	GLUDy
	TKT2	PFL	ME2
		PYK	PFL
			PYK

The knockouts are listed by their reaction names in the iAF1260 genome-scale model of E. coli metabolism.

ISSN: 1472-6750