Figure 14

Dot blot assay of AP/α-CEA-λ phage. A 1 μl spot of each of the 4 dilutions of the wild type λKM4 and AP-α-CEA-λ phage displaying AP as a fusion to the C-terminus of the gpD and anti-CEA scFv antibody as a fusion to the C-terminus of the truncated gpV, was applied onto the marked squares of a nitrocellulose filter. Then the filter was air dried approximately for 5 min and developed by incubating directly with the AP chromogenic substrate. Phage type is indicated on the left side, phage quantity in spot on the top of the figure.