Purification steps | Total protein | Total activity | Specific activity | Purification fold | Yield |
---|---|---|---|---|---|
(mg) | (U) | (U/mg) | (%) | ||
Culture broth | 101.7 | 8760001 | 8611 | 1 | 100 |
(NH4)2SO4 precipitation | 24.7 | 4300001 | 17430 | 2 | 49 |
DEAE-sepharose Fast Flow | 6 | 526240 | 87120 | 10 | 6 |
Sephadex G-100 | 1 | 501764 | 380124 | 44 | 5.7 |