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Table 1 Purification results of the phytase from a newly isolated strain B. nealsonii ZJ0702

From: Purification and characterization of a novel neutral and heat-tolerant phytase from a newly isolated strain Bacillus nealsoniiZJ0702

Purification steps

Total protein

Total activity

Specific activity

Purification fold

Yield

(mg)

(U)

(U/mg)

(%)

Culture broth

101.7

8760001

8611

1

100

(NH4)2SO4 precipitation

24.7

4300001

17430

2

49

DEAE-sepharose Fast Flow

6

526240

87120

10

6

Sephadex G-100

1

501764

380124

44

5.7