Figure 1From: Purification and characterization of a novel neutral and heat-tolerant phytase from a newly isolated strain Bacillus nealsoniiZJ0702Elution curves of the phytase from B. nealsonii ZJ0702 and the SDS-PAGE analysis. (a) The elution curve of the DEAE-sepharose anion-exchange column chromatography purification step of the sample. (b) The elution curve of the Sephadex G-100 size-exclusion chromatography purification step of the sample. (c) The SDS-PAGE analysis of the phytase. M: protein molecular weight markers; lane 1: the sample from the crude extract; lane 2: the sample after DEAE-sepharose anion-exchange column chromatography; lane 3: the sample after Sephadex G-100 size-exclusion chromatography.Back to article page